Title | Dispensability of surfactant proteins A and D in immune control of Mycobacterium tuberculosis infection following aerosol challenge of mice. |
Publication Type | Journal Article |
Year of Publication | 2011 |
Authors | Lemos MP, McKinney J, Rhee KY |
Journal | Infect Immun |
Volume | 79 |
Issue | 3 |
Pagination | 1077-85 |
Date Published | 2011 Mar |
ISSN | 1098-5522 |
Keywords | Animals, CD4-Positive T-Lymphocytes, Cell Separation, Disease Models, Animal, Flow Cytometry, Macrophages, Mice, Mice, Knockout, Mycobacterium tuberculosis, Pulmonary Surfactant-Associated Protein A, Pulmonary Surfactant-Associated Protein D, Reverse Transcriptase Polymerase Chain Reaction, Tuberculosis, Pulmonary |
Abstract | Surfactant proteins A and D (SP-A and -D) play a role in many acute bacterial, viral, and fungal infections and in acute allergic responses. In vitro, human SPs bind Mycobacterium tuberculosis and alter human and rat macrophage-mediated functions. Here we report the roles of SP-A and SP-D in M. tuberculosis infection following aerosol challenge of SP-A-, SP-D-, and SP-A/-D-deficient mice. These studies surprisingly identified no gross defects in uptake or immune control of M. tuberculosis in SP-A-, SP-D-, and SP-A/-D-deficient mice. While both SP-A- and SP-D-deficient mice exhibited evidence of immunopathologic defects, the CD11b(high) CD11c(high) dendritic cell populations and the gamma interferon (IFN-γ)-dependent CD4(+) T cell response to M. tuberculosis were unaltered in all genotypes tested. Together, these data indicate that SP-A and SP-D are dispensable for immune control of M. tuberculosis in a low-dose, aerosol challenge, murine model of tuberculosis (TB). |
DOI | 10.1128/IAI.00286-10 |
Alternate Journal | Infect Immun |
PubMed ID | 21199913 |
PubMed Central ID | PMC3067487 |