Title | Metabolomics of Mycobacterium tuberculosis. |
Publication Type | Journal Article |
Year of Publication | 2015 |
Authors | Nandakumar M, Prosser GA, de Carvalho LPedro S, Rhee K |
Journal | Methods Mol Biol |
Volume | 1285 |
Pagination | 105-15 |
Date Published | 2015 |
ISSN | 1940-6029 |
Keywords | Chromatography, Liquid, Mass Spectrometry, Metabolome, Metabolomics, Mycobacterium tuberculosis |
Abstract | Enzymes fuel the biochemical activities of all cells. Their substrates and products thus offer a potential window into the physiologic state of a cell. Metabolomics focuses on the global, or systems-level, study of small molecules in a given biological system and thus provided an experimental tool with which to study cellular physiology on a global biochemical scale. While metabolomic studies of Mycobacterium tuberculosis are still in their infancy, recent studies have begun to deliver unique insights into the composition, organization, activity, and regulation of M. tuberculosis' physiologic network. Here, we outline practical methods for the culture, collection, and analysis of metabolomic samples from Mycobacterium tuberculosis that emphasize minimal sample perturbation, broad and native metabolite recovery, and sensitive, biologically agnostic metabolite detection. |
DOI | 10.1007/978-1-4939-2450-9_6 |
Alternate Journal | Methods Mol Biol |
PubMed ID | 25779312 |