Suitability of silica hydride stationary phase, aqueous normal phase chromatography for untargeted metabolomic profiling of Enterococcus faecium and Staphylococcus aureus.

TitleSuitability of silica hydride stationary phase, aqueous normal phase chromatography for untargeted metabolomic profiling of Enterococcus faecium and Staphylococcus aureus.
Publication TypeJournal Article
Year of Publication2009
AuthorsWeisenberg SA, Butterfield TR, Fischer SM, Rhee KY
JournalJ Sep Sci
Volume32
Issue13
Pagination2262-5
Date Published2009 Jul
ISSN1615-9314
KeywordsBacterial Proteins, Chromatography, Drug Resistance, Bacterial, Enterococcus faecium, Mass Spectrometry, Metabolome, Metabolomics, Silicates, Staphylococcus aureus, Water
Abstract

We report the robustness of silica hydride stationary phase, aqueous normal phase (ANP) chromatography to the chemical complexity of the intracellular metabolomes of Staphylococcus aureus and Enterococcus faecium. We specifically demonstrate that the chromatographic behavior of known metabolites is unaffected by the intracellular chemical matrix of these microbes and that this method enables untargeted profiling of their intracellular metabolites using accurate mass-retention time (AMRT) identifiers. We further demonstrate the ability of AMRT-based metabolite profiling to differentiate bacteria along genetic and phenotypic lines. Overall, these data commend the utility of ANP-based chromatography for untargeted metabolomics-based studies of microbial physiology and antibiotic resistance.

DOI10.1002/jssc.200900256
Alternate JournalJ Sep Sci
PubMed ID19569105
PubMed Central IDPMC5821128
Grant ListUL1 RR024996 / RR / NCRR NIH HHS / United States
UL1-PRO24385 / / PHS HHS / United States